pET32bplus_trx_dAChE4 | Addgene 83917 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pET32bplus_trx_dAChE4

catalog :

83917

citations: 1

Reference
Goldenzweig A, Goldsmith M, Hill S, Gertman O, Laurino P, Ashani Y, et al. Automated Structure- and Sequence-Based Design of Proteins for High Bacterial Expression and Stability. Mol Cell. 2016;63:337-346 pubmed publisher

product information

Catalog Number :

83917

Product Name :

pET32bplus_trx_dAChE4

article :

doi	10.1016/j.molcel.2016.06.012
id	22346
pubmed_id	27425410

bacterial resistance :

Ampicillin

cloning :

backbone

pET32b+

backbone_mutation

addition of three nucleotides, GTT, at 7339-7441

backbone_origin

Novagen

backbone_size

5851

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth strain :

Encodes an E. coli codon optimized version of dAChE4 - a designed variant of human acetylcholinesterase bearing 51 mutations with ~1800 fold higher functional expression levels in E. coli

origin :

pi :

alt_names

dAChE4

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	NcoI
promoter	T7 promoter
sequencing_primer_3	caaaaaacccctcaagacccg
sequencing_primer_5	aaattcgaacgccagcacatgg
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ACEE, ARACHE, N-ACHE, YT
gene	ACHE
id	43

genbank_ids

M55040.1

mutation

V13T,K24T,M43V,L49R,V61W,S68N,E92N,T110K,S111N,T113A,L116M,A128S,Q141R,A142T,T145V,V188I,V227I,G235A,T239Y,G241S,M242R,G243E,T250L,H254K,T276N,A279P,V281E,S310P,A319N,H323K,Q326D,V332N,A358E,A362E,V379I,R394K,L395N,E397D,V409I,L415F,G417Q,L419Y,Q422N,A435S,S439P,L442E,A468K,Q475R,G507D,A508E,Q510K

name

Designed hAChE, codon optimised for E.coli expression

shRNA_sequence

size

1653

species

9606

Homo sapiens

100

Synthetic