This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET32bplus_trxAChEwt
catalog :
83916
citations: 1
Reference
Goldenzweig A, Goldsmith M, Hill S, Gertman O, Laurino P, Ashani Y, et al. Automated Structure- and Sequence-Based Design of Proteins for High Bacterial Expression and Stability. Mol Cell. 2016;63:337-346 pubmed publisher
product information
Catalog Number :
83916
Product Name :
pET32bplus_trxAChEwt
article :
doi10.1016/j.molcel.2016.06.012
id22346
pubmed_id27425410
bacterial resistance :
Ampicillin
cloning :
backbonepET32b+
backbone_mutation
backbone_originNovagen
backbone_size5848
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Encodes an E. coli codon optimized version of wild type human acetylcholinesterase
origin :
37
pi :
alt_names
hAChE-wt
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NcoI
promoterT7 promoter
sequencing_primer_3caaaaaacccctcaagacccg
sequencing_primer_5aaattcgaacgccagcacatgg
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesACEE, ARACHE, N-ACHE, YT
geneACHE
id43
genbank_ids
M55040.1
mutationcodon optimised for E.coli expression
nameShort form of hAChE, codon optimised for E.coli expression
shRNA_sequence
size1653
species
9606
Homo sapiens
100
Synthetic
tags
locationN terminal on backbone
tagTRX
locationN terminal on backbone
tagHis
locationN terminal on backbone
tagS-tag
resistance markers :
2738
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA