This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLJM5-C234S PHGDH
catalog :
83902
citations: 2
Reference
Białopiotrowicz E, Noyszewska Kania M, Kachamakova Trojanowska N, Łoboda A, Cybulska M, Grochowska A, et al. Serine Biosynthesis Pathway Supports MYC-miR-494-EZH2 Feed-Forward Circuit Necessary to Maintain Metabolic and Epigenetic Reprogramming of Burkitt Lymphoma Cells. Cancers (Basel). 2020;12: pubmed publisher
Pacold M, Brimacombe K, Chan S, Rohde J, Lewis C, Swier L, et al. A PHGDH inhibitor reveals coordination of serine synthesis and one-carbon unit fate. Nat Chem Biol. 2016;12:452-8 pubmed publisher
product information
Catalog Number :
83902
Product Name :
pLJM5-C234S PHGDH
article :
doi10.1038/nchembio.2070
id22045
pubmed_id27110680
bacterial resistance :
Ampicillin
cloning :
backbonepLJM5
backbone_mutation
backbone_origin
backbone_size8010
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
stable overexpression
origin :
37
pi :
alt_names
PHGDH
cloning
clone_methodRestriction Enzyme
cloning_site_3KpnI
cloning_site_5NheI
promoterCMV
sequencing_primer_3TAG TTT GTA TGT CTG TTG CTA TTA
sequencing_primer_5ATG TCG TAA CAA CTC CGC CCC ATT
site_3_destroyed
site_5_destroyed
entrez_gene
aliases3-PGDH, 3PGDH, HEL-S-113, NLS, NLS1, PDG, PGAD, PGD, PGDH, PHGDHD, SERA
genePHGDH
id26227
genbank_ids
NM_006623.3
mutationquikchanged cysteine 234 to serine
namePhosphoglycerate dehydrogenase
shRNA_sequence
size1602
species
9606
Homo sapiens
tags
resistance markers :
432
tags :
Unknown
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA