This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBiex PKC - Peptide 14 mCit mCer
catalog :
83565
citations: 1
Reference
Sommese R, Sivaramakrishnan S. Substrate Affinity Differentially Influences Protein Kinase C Regulation and Inhibitor Potency. J Biol Chem. 2016;291:21963-21970 pubmed
product information
Catalog Number :
83565
Product Name :
pBiex PKC - Peptide 14 mCit mCer
article :
doi10.1074/jbc.M116.737601
id22303
pubmed_id27555323
bacterial resistance :
Ampicillin
cloning :
backbonepBiex-1
backbone_mutation
backbone_originNovagen
backbone_size5259
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
Insect Expression
growth strain :
Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the pseudosubstrate of PKCalpha.
origin :
37
pi :
alt_names
PRKCA
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5HindIII
promoterT7 Promoter
sequencing_primer_3T7 Reverse
sequencing_primer_5T7 Foward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAAG6, PKC-alpha, PKCA, PKCI+/-, PKCalpha, PRKACA
genePRKCA
id5578
genbank_ids
mutationOnly catalytic domain of PKC (aa 335-672)
namePKC alpha
shRNA_sequence
size2892
species
9606
Homo sapiens
tags
location
tagmCitrine
location
tagmCerulean
location
tagTev Protease Site
location
tag10 nm ER/K Helix
locationC terminal on insert
tagFLAG Purificaiton Tag
resistance markers :
1521
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA