pPHAGE_DLD-H450A-C-TAP | Addgene 83478 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pPHAGE_DLD-H450A-C-TAP

catalog :

83478

citations: 1

Reference
Malty R, Aoki H, Kumar A, Phanse S, Amin S, Zhang Q, et al. A Map of Human Mitochondrial Protein Interactions Linked to Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and NF-κB Signaling. Cell Syst. 2017;5:564-577.e12 pubmed publisher

product information

Catalog Number :

83478

Product Name :

pPHAGE_DLD-H450A-C-TAP

article :

doi	10.1016/j.cels.2017.10.010
id	28192374
pubmed_id	29128334

bacterial resistance :

Ampicillin

cloning :

backbone

pPHAGE C-TAP

backbone_mutation

Partial removal of gateway cassette.

backbone_origin

PMID: 26186194

backbone_size

10056

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

Mutation (first described in PMID: 17404228) was created using the method described in PMID: 17446874. Two outer (F: CTCTTGGTTTGCATTGGCCG, IRES_R: CCTCACATTGCCAAAAGACG) and two mutation-site primers were used. The mutated PCR product and the backbone vector were digested using EcoRI, gel purified and ligated. Note that the mutation is numbered in accordance with the original publication (PMID: 17404228), which lacked the mitochondrial targeting signal (residues 1-35). In this full-length protein, it is found at position 485.

growth strain :

Mammalian expression of the dehydrogenase-deficient mutant DLD-H450A.

origin :

pi :

alt_names

Dihydrolipoamide Dehydrogenase

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter	CMV
sequencing_primer_3	IRES_R
sequencing_primer_5	CMV_F, mutation confirmation (CTCTTGGTTTGCATTGGCCG)
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	DLDD, DLDH, E3, GCSL, LAD, OGDC-E3, PHE3
gene	DLD
id	1738

genbank_ids

NM_000108.4

mutation

H450A

name

DLD

shRNA_sequence

size

species

9606

Homo sapiens