This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLEX_307-SOD1WT
catalog :
83444
citations: 1
Reference
Malty R, Aoki H, Kumar A, Phanse S, Amin S, Zhang Q, et al. A Map of Human Mitochondrial Protein Interactions Linked to Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and NF-κB Signaling. Cell Syst. 2017;5:564-577.e12 pubmed publisher
product information
Catalog Number :
83444
Product Name :
pLEX_307-SOD1WT
article :
doi10.1016/j.cels.2017.10.010
id28192374
pubmed_id29128334
bacterial resistance :
Ampicillin
cloning :
backbonepLEX_307 (plasmid #41392)
backbone_mutationRemoval of gateway cloning cassette and V5 tag
backbone_originDavid Root
backbone_size10065
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
PCR cloning (F: TAAGCAGCTAGCAGCTCAGATCTCGAGCTCAAGC, R: TAAGCAACTAGTTCATTGGGCGATCCCAATTACACC) followed by cleanup, NheI/SpeI restriction digestion of PCR product and destination vector, gel purification and ligation.
growth strain :
Mammalian expression of SOD1WT
growth temp :
No longer than 16 hours culture.
origin :
30
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3SpeI
cloning_site_5NheI
promoterEF-1
sequencing_primer_3WPRE_R
sequencing_primer_5EF-1 _F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesALS, ALS1, HEL-S-44, IPOA, SOD, STAHP, hSod1, homodimer
geneSOD1
id6647
genbank_ids
mutation
nameSOD1
shRNA_sequence
size720
species
9606
Homo sapiens
tags
plasmid copy :
Elizabeth Fisher( pF147 pSOD1A4VAcGFP1, #26408)
resistance markers :
3055
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA