This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSOD1E133 -AcGFP1
catalog :
83419
citations: 1
Reference
Malty R, Aoki H, Kumar A, Phanse S, Amin S, Zhang Q, et al. A Map of Human Mitochondrial Protein Interactions Linked to Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and NF-κB Signaling. Cell Syst. 2017;5:564-577.e12 pubmed publisher
product information
Catalog Number :
83419
Product Name :
pSOD1E133 -AcGFP1
article :
doi10.1016/j.cels.2017.10.010
id28192374
pubmed_id29128334
bacterial resistance :
Kanamycin
cloning :
backbonepAcGFP1-N1
backbone_mutation
backbone_originClontech
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Mutation (first described in PMID: 8938700) was created using the method described in PMID: 17446874. Two outer and two mutation-site primers were used. The mutated PCR product and the backbone vector were digested using EcoRI and SalI, gel purified and ligated.
growth strain :
Expresses SOD1E133 in mammalian cells.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5EcoRI
promoterCMV
sequencing_primer_3AcGFP1_R (GGCCATTCACATCGCCATTC)
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesALS, ALS1, HEL-S-44, IPOA, SOD, STAHP, hSod1, homodimer
geneSOD1
id6647
genbank_ids
mutationE133 deletion
nameSOD1
shRNA_sequence
size720
species
9606
Homo sapiens
tags
locationC terminal on insert
tagAcGFP1
plasmid copy :
Elizabeth Fisher( pF147 pSOD1A4VAcGFP1, #26408)
resistance markers :
3055
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA