This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCDNA5_FRB-pBirA-HA
catalog :
83415
citations: 1
Reference
Lee S, Lee H, Lee H, Lee S, Ha S, Kwon T, et al. Proximity-Directed Labeling Reveals a New Rapamycin-Induced Heterodimer of FKBP25 and FRB in Live Cells. ACS Cent Sci. 2016;2:506-16 pubmed publisher
product information
Catalog Number :
83415
Product Name :
pCDNA5_FRB-pBirA-HA
article :
doi10.1021/acscentsci.6b00137
id22244
pubmed_id27610411
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA5
backbone_mutation
backbone_origin
backbone_size5082
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
proximity-directed biotin labeling on the FRB domain of mTOR in mammalian cell
origin :
37
pi :
alt_names
FKBP12-rapamycin binding domain
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5KpnI
promoterCMV-F
sequencing_primer_3BGH-R
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesFRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS
geneMTOR
id2475
genbank_ids
NM_004958.3.
mutation
nameFRB-pBirA-HA
shRNA_sequence
size1302
species
9606
Homo sapiens
100
Synthetic
tags
locationC terminal on insert
tagpBirA
locationC terminal on insert
tagHA
resistance markers :
3220
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA