This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pADtet-His6-MscL
catalog :
83373
citations: 2
Reference
Jacobs M, Boyd M, Kamat N. Diblock copolymers enhance folding of a mechanosensitive membrane protein during cell-free expression. Proc Natl Acad Sci U S A. 2019;: pubmed publisher
Heureaux J, Chen D, Murray V, Deng C, Liu A. Activation of a bacterial mechanosensitive channel in mammalian cells by cytoskeletal stress. Cell Mol Bioeng. 2014;7:307-319 pubmed
product information
Catalog Number :
83373
Product Name :
pADtet-His6-MscL
article :
doi10.1007/s12195-014-0337-8
id22231
pubmed_id25606062
bacterial resistance :
Ampicillin
cloning :
backbonepADtet
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Adenoviral
growth strain :
Expresses wild type MscL in mammalian cells
origin :
37
pi :
alt_names
MscL
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3SV40 polyA
sequencing_primer_5CMVmin
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesb3291, ECK3277, yhdC
genemscL
id947787
genbank_ids
NC_000913.3
mutation
nameMechanosensitive channel of large conductance
shRNA_sequence
size408
species
32644
Other
E. coli
tags
locationN terminal on insert
tagHis6 tag
plasmid copy :
Dr. Boris Martinac from Victor Chang Cardiac Research Institute in Australia
resistance markers :
3047
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA