This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSc-His6-Sem1Sc
catalog :
83235
citations: 1
Reference
Levin Kravets O, Tanner N, Shohat N, Attali I, Keren Kaplan T, Shusterman A, et al. A bacterial genetic selection system for ubiquitylation cascade discovery. Nat Methods. 2016;13:945-952 pubmed publisher
product information
Catalog Number :
83235
Product Name :
pSc-His6-Sem1Sc
article :
doi10.1038/nmeth.4003
id22790
pubmed_id27694912
bacterial resistance :
Ampicillin
cloning :
backbonepGREG600
backbone_mutationTo create the pScHis6Sem1 the GFP sequence was removed from the pGREG600 vector by Gibson assembly (Gibson et al, 2009). Then the cDHA of yeast Sem1 was sub-cloned in frame with His6 under the Gal promoter.
backbone_origin
backbone_size7176
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth strain :
pGREG600-His6-Sem1Sc, Expresses the His6-Sem1 in yeast
origin :
37
pi :
alt_names
cloning
entrez_gene
aliasesYDR363W-A, DSS1, HOD1
geneSEM1
id851967
genbank_ids
mutation
nameSem 1
shRNA_sequence
size270
species
4932
Saccharomyces cerevisiae
tags
locationN terminal on insert
tagHis6
resistance markers :
3036
tags :
Unknown
terms :
Neomycin (select with G418)
URA3
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA