pGEN6-Ubc5 Sc | Addgene 83234 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pGEN6-Ubc5 Sc

catalog :

83234

citations: 1

Reference
Levin Kravets O, Tanner N, Shohat N, Attali I, Keren Kaplan T, Shusterman A, et al. A bacterial genetic selection system for ubiquitylation cascade discovery. Nat Methods. 2016;13:945-952 pubmed publisher

product information

Catalog Number :

83234

Product Name :

pGEN6-Ubc5 Sc

article :

doi	10.1038/nmeth.4003
id	22790
pubmed_id	27694912

bacterial resistance :

Kanamycin

cloning :

backbone

pHIS parallele 2

backbone_mutation

The pND-Ub vector is based on a modified pZE21 vector, contacting the pLtetO-1. cDNAs were amplified by polymerase chain reaction (PCR), digested and sub-cloned into the vectors. The mouse cDNA of the nDHFR fragment (residues 1-108) was PCR-amplified and sub-cloned between the KpnI and PacI sites (the PacI site was inserted into the vector by PCR) into the pZE21 vector. Then a flexible linker (Figure 4) was constructed a using two-step PCR method and digested with PacI and NotI (the NotI site was inserted into the vector by PCR) and cloned into the vector downstream to the pLtetO1-nDHFR. Ubiquitylation apparatus cassettes containing a His6-Ub-E2-E1 were PCR-amplified from pGEN plasmids expressing different E2s or Ub mutants (Keren-Kaplan et al, 2011) and sub-cloned as fusion to the C-terminus of the nDHFR-linker1 into the NotI and AvrII endonuclease recognition sites.

backbone_origin

backbone_size

5289

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth strain :

pHis-par2-UbHs/Ubc5Sc/ Uba1Ta

origin :

pi :

alt_names

Ubq

cloning

entrez_gene

aliases	HMG20
gene	UBC
id	7316

genbank_ids

mutation

name

Ubiqutin

shRNA_sequence

size

231

species

9606

Homo sapiens