This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGEX-4T2 SHP2 D61A
catalog :
8323
citations: 1
Reference
O Reilly A, Pluskey S, Shoelson S, Neel B. Activated mutants of SHP-2 preferentially induce elongation of Xenopus animal caps. Mol Cell Biol. 2000;20:299-311 pubmed
product information
Catalog Number :
8323
Product Name :
pGEX-4T2 SHP2 D61A
article :
doi10.1128/MCB.20.1.299-311.2000
id152
pubmed_id10594032
bacterial resistance :
Ampicillin
cloning :
backbonepGEX-4T-2
backbone_mutation
backbone_origin
backbone_size4970
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Back-side mutation of SHP2--activating in Xenopus and against RCM-lysozyme. Can still bind peptide through SH2 domain, further activated in vitro by peptide engagement.
origin :
37
pi :
alt_names
SH-PTP2
PTPN11
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoR1
cloning_site_5EcoRI
promoter
sequencing_primer_3
sequencing_primer_5na
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBPTP3, CFC, JMML, METCDS, NS1, PTP-1D, PTP2C, SH-PTP2, SH-PTP3, SHP2
genePTPN11
id5781
genbank_ids
L03535
mutationD61A (activated mutant)
nameSHP2
shRNA_sequence
size2276
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagGST
resistance markers :
358
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA