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product summary
company name :
Addgene
product type :
cDNA
product name :
pND-Ub03-CDC34 Sc
catalog :
83209
citations: 1
product information
Catalog Number :
83209
Product Name :
pND-Ub03-CDC34 Sc
article :
| doi | 10.1038/nmeth.4003 |
| id | 22790 |
| pubmed_id | 27694912 |
bacterial resistance :
Kanamycin
cloning :
| backbone | pZE21 | |
| backbone_mutation | The pND-Ub vector is based on a modified pZE21 vector, contacting the pLtetO-1. cDNAs were amplified by polymerase chain reaction (PCR), digested and sub-cloned into the vectors. The mouse cDNA of the nDHFR fragment (residues 1-108) was PCR-amplified and sub-cloned between the KpnI and PacI sites (the PacI site was inserted into the vector by PCR) into the pZE21 vector. Then a flexible linker (Figure 4) was constructed a using two-step PCR method and digested with PacI and NotI (the NotI site was inserted into the vector by PCR) and cloned into the vector downstream to the pLtetO1-nDHFR. Ubiquitylation apparatus cassettes containing a His6-Ub-E2-E1 were PCR-amplified from pGEN plasmids expressing different E2s or Ub mutants (Keren-Kaplan et al, 2011) and sub-cloned as fusion to the C-terminus of the nDHFR-linker1 into the NotI and AvrII endonuclease recognition sites. | |
| backbone_origin | ||
| backbone_size | 2219 | |
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
description :
Expresses the nDHFR-link1-His6-Ub, CDC34 Sc and UBA1Ta in E.coli
growth temp :
37
inserts :
|
pi :
| 3036 |
plasmid copy :
High Copy
terms :
MTA
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments