This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLX304 PCSK5 (A270T)-V5 blast
catalog :
83102
citations: 1
Reference
Bajikar S, Wang C, Borten M, Pereira E, Atkins K, Janes K. Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor Sequestration in Triple-Negative Breast Cancer. Dev Cell. 2017;43:418-435.e13 pubmed publisher
product information
Catalog Number :
83102
Product Name :
pLX304 PCSK5 (A270T)-V5 blast
article :
doi10.1016/j.devcel.2017.10.027
id22167
pubmed_id29161592
bacterial resistance :
Ampicillin
cloning :
backbonepLX304
backbone_mutation
backbone_originAddgene #25890
backbone_size9377
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
Plasmid for stable expression of human PC5A (PCSK5) mutant A270T with C-terminal V5 tag. Wild-type PCSK5 sequence was mutated with QuikChange Site Directed Mutagenesis.
growth strain :
Lentiviral vector for constitutive expression of human mutant PC5A (A270T) with C-terminal V5 tag
origin :
37
pi :
alt_names
PC5A
PC5
SPC6
cloning
entrez_gene
aliasesPC5, PC6, PC6A, SPC6
genePCSK5
id5125
genbank_ids
mutationChanged Alanine 270 to Threonine
namePCSK5
shRNA_sequence
size2742
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagV5
plasmid copy :
Thermo Open Biosystems Human ORFeome version 5.1 #7239
resistance markers :
1585
tags :
Low Copy
terms :
Blasticidin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA