This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEN_TT 3xFLAG SMAD4
catalog :
83094
citations: 1
Reference
Bajikar S, Wang C, Borten M, Pereira E, Atkins K, Janes K. Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor Sequestration in Triple-Negative Breast Cancer. Dev Cell. 2017;43:418-435.e13 pubmed publisher
product information
Catalog Number :
83094
Product Name :
pEN_TT 3xFLAG SMAD4
article :
doi10.1016/j.devcel.2017.10.027
id22167
pubmed_id29161592
bacterial resistance :
Gentamicin
cloning :
backbonepEN_TTmiRc2
backbone_mutation3xFLAG cloned in frame before restriction sites
backbone_originAddgene #83274
backbone_size4422
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
entry vector for gateway cloning
growth notes :
Gateway entry vector for expression of 3xFLAG SMAD4
growth strain :
Gateway entry vector for inducible expression of human SMAD4 with N-terminal 3xFLAG tag
growth temp :
DB3.1 (ccdB survival)
origin :
37
pi :
alt_names
DPC4
MADH4
SMAD family member 4
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5SpeI
promoterTRE tight promoter
sequencing_primer_3
sequencing_primer_5pCEP-fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDPC4, JIP, MADH4, MYHRS
geneSMAD4
id4089
genbank_ids
NM_005359
mutation
nameSMAD4
shRNA_sequence
size1659
species
9606
Homo sapiens
tags
locationN terminal on backbone
tag3xFLAG
plasmid copy :
PCR cloned from Thermo Open Biosystems Human ORFeome version 5.1 #4687
resistance markers :
1585
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA