This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
TET-pLKO.1 PURO shSMAD4 #2
catalog :
83091
citations: 1
Reference
Bajikar S, Wang C, Borten M, Pereira E, Atkins K, Janes K. Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor Sequestration in Triple-Negative Breast Cancer. Dev Cell. 2017;43:418-435.e13 pubmed publisher
product information
Catalog Number :
83091
Product Name :
TET-pLKO.1 PURO shSMAD4 #2
article :
doi10.1016/j.devcel.2017.10.027
id22167
pubmed_id29161592
bacterial resistance :
Ampicillin
cloning :
backboneTet-pLKO-puro
backbone_mutation
backbone_originAddgene #21915
backbone_size10633
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
The targeting sequence was identified from the TRC (TRCN0000288653). Potently knocks down SMAD4 expression after 3 x 500 uL lentiviral infection in MDA-MB-231 cells.
growth strain :
Lentiviral shRNA vector for inducible knockdown of human SMAD4
origin :
30
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5AgeI
promoter
sequencing_primer_3
sequencing_primer_5GGCAGGGATATTCACCATTATCGTTTCAGA
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDPC4, JIP, MADH4, MYHRS
geneSMAD4
id4089
genbank_ids
NM_005359
mutation
nameshSMAD4
shRNA_sequenceGCAGACAGAAACTGGATTAAA
size
species
9606
Homo sapiens
tags
resistance markers :
1585
tags :
Low Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA