This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
TET-pLKO.1 PURO shID2 #2
catalog :
83087
citations: 2
Reference
Koppenhafer S, Goss K, Voigt E, Croushore E, Terry W, Ostergaard J, et al. Inhibitor of DNA binding 2 (ID2) regulates the expression of developmental genes and tumorigenesis in ewing sarcoma. Oncogene. 2022;41:2873-2884 pubmed publisher
Bajikar S, Wang C, Borten M, Pereira E, Atkins K, Janes K. Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor Sequestration in Triple-Negative Breast Cancer. Dev Cell. 2017;43:418-435.e13 pubmed publisher
product information
Catalog Number :
83087
Product Name :
TET-pLKO.1 PURO shID2 #2
article :
doi10.1016/j.devcel.2017.10.027
id22167
pubmed_id29161592
bacterial resistance :
Ampicillin
cloning :
backboneTet-pLKO-puro
backbone_mutation
backbone_originAddgene #21915
backbone_size10633
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
The targeting sequence was identified from the TRC (TRCN0000232079). Knockdown validated by Western blot of transient, ectopic expression of ID2 in 293T cells.
growth strain :
Lentiviral shRNA vector for inducible knockdown of human ID2
origin :
30
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3E
cloning_site_5AgeI
promoter
sequencing_primer_3
sequencing_primer_5GGCAGGGATATTCACCATTATCGTTTCAGA
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesGIG8, ID2A, ID2H, bHLHb26
geneID2
id3398
genbank_ids
NM_002166
mutation
nameshID2
shRNA_sequenceGACCACCCTCAACACGGATAT
size
species
9606
Homo sapiens
tags
resistance markers :
1585
tags :
Low Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA