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product summary
company name :
Addgene
product type :
cDNA
product name :
pCD-Sub74-Ent1 (1-152) E41R,E44R Sc
catalog :
83072
citations: 1
product information
Catalog Number :
83072
Product Name :
pCD-Sub74-Ent1 (1-152) E41R,E44R Sc
article :
| doi | 10.1038/nmeth.4003 |
| id | 22790 |
| pubmed_id | 27694912 |
bacterial resistance :
Spectinomycin
cloning :
| backbone | pCDFDuet-1 | |
| backbone_mutation | The pCD-Sub vector was constructed based on a pCDF-duet vector. The T7 promoter was substituted with pLtetO-1. The cDHFR fragment (residues 109-187) was PCR-amplified and sub-cloned under the pLtetO1 promoter at the MluI and AscI endonuclease recognition sites (the AscI site was inserted by PCR). A second linker (linker2; as indicated in Figure 4B); was fused to the N-terminus of MBP (Maltose Binding Protein) and cloned into the AscI site. Substrates were PCR-amplified and sub-cloned into SacII and SpeI sites downstream and in-frame with the cDHFR-linker2-MBP. In some vectors the MBP was removed. Some vectors were prepared by complete chemical synthesis assembly (Gibson et al, 2009). | |
| backbone_origin | ||
| backbone_size | 4929 | |
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
description :
Expresses the cDHFR-link2-His6-Ent1 (1-152) E41R,E44R Sc in E.coli
growth temp :
37
inserts :
|
pi :
| 3036 |
plasmid copy :
Unknown
terms :
MTA
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments