pDONR223_CDC45_WT_V5 | Addgene 82990 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pDONR223_CDC45_WT_V5

catalog :

82990

citations: 3

Reference
Zonderland G, Vanzo R, Gadi S, Mart xed n Doncel E, Coscia F, Mund A, et al. The TRESLIN-MTBP complex couples completion of DNA replication with S/G2 transition. Mol Cell. 2022;82:3350-3365.e7 pubmed publisher
Zhang R, Liu Z, Zhang G. CDC45 modulates MCM7 expression and inhibits cell proliferation by suppressing the PI3K/AKT pathway in acute myeloid leukemia. Am J Transl Res. 2021;13:10218-10232 pubmed
Berger A, Brooks A, Wu X, Shrestha Y, Chouinard C, Piccioni F, et al. High-throughput Phenotyping of Lung Cancer Somatic Mutations. Cancer Cell. 2016;30:214-228 pubmed publisher

product information

Catalog Number :

82990

Product Name :

pDONR223_CDC45_WT_V5

article :

doi	10.1016/j.ccell.2016.06.022
id	25588
pubmed_id	27478040

bacterial resistance :

Spectinomycin

cloning :

backbone

pDONR223

backbone_mutation

backbone_origin

Invitrogen

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

Gateway Entry vector

growth notes :

Open state: NO stop codon immediately follows insert

growth strain :

Gateway Donor vector containing CDC45, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

origin :

pi :

alt_names

NM_003504

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter	None
sequencing_primer_3	M13-Rev
sequencing_primer_5	M13pUC-fwd
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CDC45L, CDC45L2, MGORS7, PORC-PI-1
gene	CDC45
id	8318

genbank_ids

mutation

name

CDC45

shRNA_sequence

size

species

9606

Homo sapiens