pDONR223_ATG3_WT_V5 | Addgene 82952 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pDONR223_ATG3_WT_V5

catalog :

82952

citations: 2

Reference
Agrotis A, von Chamier L, Oliver H, Kiso K, Singh T, Ketteler R. Human ATG4 autophagy proteases counteract attachment of ubiquitin-like LC3/GABARAP proteins to other cellular proteins. J Biol Chem. 2019;294:12610-12621 pubmed publisher
Berger A, Brooks A, Wu X, Shrestha Y, Chouinard C, Piccioni F, et al. High-throughput Phenotyping of Lung Cancer Somatic Mutations. Cancer Cell. 2016;30:214-228 pubmed publisher

product information

Catalog Number :

82952

Product Name :

pDONR223_ATG3_WT_V5

article :

doi	10.1016/j.ccell.2016.06.022
id	25588
pubmed_id	27478040

bacterial resistance :

Spectinomycin

cloning :

backbone

pDONR223

backbone_mutation

backbone_origin

Invitrogen

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

Gateway Entry vector

growth notes :

Open state: NO stop codon immediately follows insert

growth strain :

Gateway Donor vector containing ATG3, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

origin :

pi :

alt_names

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter	None
sequencing_primer_3	M13-Rev
sequencing_primer_5	M13pUC-fwd
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	APG3, APG3-LIKE, APG3L, PC3-96, hApg3
gene	ATG3
id	64422

genbank_ids

NM_022488

mutation

name

ATG3

shRNA_sequence

size

species

9606

Homo sapiens