This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDONR223_PIK3CA_p.E545K
catalog :
82881
citations: 3
Reference
Ferrarone J, Thomas J, Unni A, Zheng Y, Nagiec M, Gardner E, et al. LKB1 suppresses growth and promotes the internalization of EGFR through the PIKFYVE lipid kinase. bioRxiv. 2023;: pubmed publisher
Lu D, Nagelberg A, Chow J, Chen Y, Michalchuk Q, Somwar R, et al. MET Exon 14 Splice-Site Mutations Preferentially Activate KRAS Signaling to Drive Tumourigenesis. Cancers (Basel). 2022;14: pubmed publisher
Berger A, Brooks A, Wu X, Shrestha Y, Chouinard C, Piccioni F, et al. High-throughput Phenotyping of Lung Cancer Somatic Mutations. Cancer Cell. 2016;30:214-228 pubmed publisher
product information
Catalog Number :
82881
Product Name :
pDONR223_PIK3CA_p.E545K
article :
doi10.1016/j.ccell.2016.06.022
id25588
pubmed_id27478040
bacterial resistance :
Spectinomycin
cloning :
backbonepDONR223
backbone_mutation
backbone_originInvitrogen
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Gateway Entry vector
growth notes :
CLOSED (stop codon immediately follows insert)
growth strain :
Gateway Donor vector containing PIK3CA, part of the Target Accelerator Plasmid Collection.
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterNone
sequencing_primer_3M13-Rev
sequencing_primer_5M13pUC-fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCCM4, CLAPO, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, PI3K-alpha, p110-alpha
genePIK3CA
id5290
genbank_ids
NM_006218
mutationE545K
nameNM_006218
shRNA_sequence
size
species
9606
Homo sapiens
tags
resistance markers :
2996
338
423
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA