This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
p.UTA.2.0 miR-27a-3p 3 perfect copies.
catalog :
82439
product information
Catalog Number :
82439
Product Name :
p.UTA.2.0 miR-27a-3p 3 perfect copies.
article :
doidoi:10.1038/srep45197
id22044
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepsiCHECK -2 Vector
backbone_mutationLuciferase genes were replaced with CFP and YFP. YFP after the Tk Promoter and CFP after te SV40 promoter. Other regions were changes to full fill correct ORF for the fluorescent proteins and full fill cloning requirements.
backbone_originPromega
backbone_size3660
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
growth notes :
Please see the following published protocol for details on plasmid use and data analysis: https://bio-protocol.org/e3000
growth strain :
Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-27a-3p in three copies
origin :
37
pi :
alt_names
cloning
entrez_gene
aliasesMIR27, MIRN27A, mir-27a
geneMIR27A
id407018
genbank_ids
NR_029501.1
mutationThree copies hsa-miR-27a-3p Complementary region as sensor
namehsa-miR-27a-3p
shRNA_sequence
size79
species
9606
Homo sapiens
tags
plasmid copy :
EYFP from pLenti-CaMKIIa-eArchT 3.0-EYFP (Plasmid #35513) ECFP from pcDNA3-CFP (Plasmid #13030)
resistance markers :
3019
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA