pFUSE2ss-CLIg-mK_M18 | Addgene 82358 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pFUSE2ss-CLIg-mK_M18

catalog :

82358

citations: 2

Reference
Klaus T, Bereta J. CH2 Domain of Mouse IgG3 Governs Antibody Oligomerization, Increases Functional Affinity to Multivalent Antigens and Enhances Hemagglutination. Front Immunol. 2018;9:1096 pubmed publisher
Klaus T, Bzowska M, Kulesza M, Kabat A, JemioÅ‚a RzemiÅ„ska M, Czaplicki D, et al. Agglutinating mouse IgG3 compares favourably with IgMs in typing of the blood group B antigen: Functionality and stability studies. Sci Rep. 2016;6:30938 pubmed publisher

product information

Catalog Number :

82358

Product Name :

pFUSE2ss-CLIg-mK_M18

article :

doi	10.1038/srep30938
id	22030
pubmed_id	27484487

bacterial resistance :

Blasticidin

cloning :

backbone

pFUSE2ss-CLIg-mK

backbone_mutation

none

backbone_origin

Invivogen

backbone_size

3862

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

Expression plasmid coding for kappa light chain of mouse antibody specific to antigen B of the ABO blood group system, clone M18.

growth temp :

Top10 or DH5alpha could be selected on LB LOW SALT with 100 micrograms/ml Blasticidin.

origin :

pi :

alt_names

Igk

cloning

clone_method	Restriction Enzyme
cloning_site_3	BstAPI
cloning_site_5	EcoRI
promoter	hEF1-HTLV prom
sequencing_primer_3	ACACTCATTCCTGTTGAAGCTC
sequencing_primer_5	AGATGTTGTTCTGACCCAAACTC
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	kappa
gene	Igk
id	243469

aliases	Igk-C
gene	Igkc
id	16071

aliases
gene	Igk-V
id	16080

genbank_ids

NC_000072.6

mutation

name

mouse immunoglobulin kappa light chain, variable fragment derived from clone M18

shRNA_sequence

size

339

species

10090

Mus musculus