This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
FLAG-SLC30A10-E25A
catalog :
82345
citations: 1
Reference
Zogzas C, Aschner M, Mukhopadhyay S. Structural Elements in the Transmembrane and Cytoplasmic Domains of the Metal Transporter SLC30A10 Are Required for Its Manganese Efflux Activity. J Biol Chem. 2016;291:15940-57 pubmed publisher
product information
Catalog Number :
82345
Product Name :
FLAG-SLC30A10-E25A
article :
doi10.1074/jbc.M116.726935
id22024
pubmed_id27307044
bacterial resistance :
Ampicillin
cloning :
backbonepCMV-FLAG10
backbone_mutation
backbone_originSigma
backbone_size6500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
See for reference to SLC30A10-WT: SLC30A10 is a cell surface-localized manganese efflux transporter, and parkinsonism-causing mutations block its intracellular trafficking and efflux activity. Leyva-Illades D, Chen P, Zogzas CE, Hutchens S, Mercado JM, Swaim CD, Morrisett RA, Bowman AB, Aschner M, Mukhopadhyay S. J Neurosci. 2014 Oct 15;34(42):14079-95. doi: 10.1523/JNEUROSCI.2329-14.2014. PMID: 25319704
growth strain :
Expresses FLAG-tagged SLC30A10-E25A in mammalian cells
origin :
37
pi :
alt_names
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3Internal SLC30A10
sequencing_primer_5CMV forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHMDPC, HMNDYT1, ZNT10, ZNT8, ZRC1, ZnT-10
geneSLC30A10
id55532
genbank_ids
mutationE25A
nameSLC30A10
shRNA_sequence
size1500
species
9606
Homo sapiens
tags
locationN terminal on backbone
tag3xFLAG
plasmid copy :
SLC30A10-WT originally obtained from Dr. Ruth Valentine. E25A mutation introduced using quick-change.
resistance markers :
2370
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA