This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMH-macroHC
catalog :
82337
citations: 1
Reference
Wild R, Hothorn M. The macro domain as fusion tag for carrier-driven crystallization. Protein Sci. 2017;26:365-374 pubmed publisher
product information
Catalog Number :
82337
Product Name :
pMH-macroHC
article :
doi10.1002/pro.3073
id22708
pubmed_id27774698
bacterial resistance :
Kanamycin
cloning :
backbonepET-24d modified
backbone_mutationInsertion of a macro domain fusion tag
backbone_originEMBL pepcore
backbone_size5502
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Provides a C-terminal macro domain and 6xHis tag for expression in E. coli
origin :
37
pi :
alt_names
VTC4
cloning
clone_methodRestriction Enzyme
cloning_site_3NcoI
cloning_site_5XbaI
promoter
sequencing_primer_3CAGCTTCCTTTCGGGCTTTGTTAGCAG
sequencing_primer_5TGCGTCCGGCGTAGAGGATCGAGATCT
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYJL012C, PHM3, YJL012C-A
geneVTC4
id853441
genbank_ids
NM_001181446
mutationTruncated protein, amino acids 1-178
nameSPX domain of the Vacuolar Transporter Chaperone 4
shRNA_sequence
size829
species
4932
Saccharomyces cerevisiae
tags
locationC terminal on backbone
tagmacro domain of human histone macroH2A1.1
locationC terminal on backbone
tag6xHis non-cleavable
resistance markers :
3008
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA