WT1-2A-eGFP PGK PuroR Donor Plasmid | Addgene 82333 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

WT1-2A-eGFP PGK PuroR Donor Plasmid

catalog :

82333

citations: 2

Reference
Chen S, Sun S, Moonen D, Lee C, Lee A, Schaffer D, et al. CRISPR-READI: Efficient Generation of Knockin Mice by CRISPR RNP Electroporation and AAV Donor Infection. Cell Rep. 2019;27:3780-3789.e4 pubmed publisher
Bao X, Lian X, Hacker T, Schmuck E, Qian T, Bhute V, et al. Long-term self-renewing human epicardial cells generated from pluripotent stem cells under defined xeno-free conditions. Nat Biomed Eng. 2016;1: pubmed publisher

product information

Catalog Number :

82333

Product Name :

WT1-2A-eGFP PGK PuroR Donor Plasmid

article :

doi	10.1038/s41551-016-0003
id	22017
pubmed_id	28462012

bacterial resistance :

Ampicillin

cloning :

backbone

OCT4-2A-eGFP

backbone_mutation

Replacing OCT4 5' and 3' arms with WT1 arms

backbone_origin

Addgene #31939

backbone_size

6804

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

CRISPR

TALEN

growth strain :

WT1 targeting donor

origin :

pi :

alt_names

Wilms tumor 1

AWT1

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	CCGGGATTCTCTTCGACAT
sequencing_primer_5	CAGGAAACAGCTATGAC
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AWT1, GUD, NPHS4, WAGR, WIT-2, WT-1, WT33
gene	WT1
id	7490

genbank_ids

Gene ID 7490

mutation

name

WT1 5' Arm

shRNA_sequence

size

1990

species

9606

Homo sapiens