pICE-HA-Ku70-siR-Mut6E | Addgene 82330 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pICE-HA-Ku70-siR-Mut6E

catalog :

82330

citations: 1

Reference
Lamaa A, Le Bras M, Skuli N, Britton S, Frit P, Calsou P, et al. A novel cytoprotective function for the DNA repair protein Ku in regulating p53 mRNA translation andÂ function. EMBO Rep. 2016;17:508-18 pubmed publisher

product information

Catalog Number :

82330

Product Name :

pICE-HA-Ku70-siR-Mut6E

article :

doi	10.15252/embr.201541181
id	22014
pubmed_id	26964895

bacterial resistance :

Ampicillin

cloning :

backbone

pICE

backbone_mutation

HA tag and cloning sites

backbone_origin

Synthetic - Addgene plasmid #46960

backbone_size

5009

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

Plasmid for constitutive or doxy-inducible expression of mutant human Ku70 unable to bind DNA and resistant to siRNA. Confers resistance to puro. Use T-REx cells for doxycycline-inducible expression.

origin :

pi :

alt_names

XRCC6

cloning

clone_method	Restriction Enzyme
cloning_site_3	NotI
cloning_site_5	XhoI
promoter	CMV-tet
sequencing_primer_3	BGH-R
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CTC75, CTCBF, G22P1, KU70, ML8, TLAA
gene	XRCC6
id	2547

genbank_ids

mutation

Mut6E corresponding to K282E K287E T300E K331E K338E R403E; mutant unable to bind to DNA

name

Ku70

shRNA_sequence

size

1830

species

9606

Homo sapiens