pDONR223_PIK3CD_WT | Addgene 82222 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pDONR223_PIK3CD_WT

catalog :

82222

citations: 3

Reference
Pemovska T, Bigenzahn J, Srndic I, Lercher A, Bergthaler A, César Razquin A, et al. Metabolic drug survey highlights cancer cell dependencies and vulnerabilities. Nat Commun. 2021;12:7190 pubmed publisher
Wu M, Xia X, Hu J, Fowlkes N, Li S. WSX1 act as a tumor suppressor in hepatocellular carcinoma by downregulating neoplastic PD-L1 expression. Nat Commun. 2021;12:3500 pubmed publisher
Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, et al. Systematic Functional Interrogation of Rare Cancer Variants Identifies Oncogenic Alleles. Cancer Discov. 2016;6:714-26 pubmed publisher

product information

Catalog Number :

82222

Product Name :

pDONR223_PIK3CD_WT

article :

doi	10.1158/2159-8290.CD-16-0160
id	22007
pubmed_id	27147599

bacterial resistance :

Spectinomycin

cloning :

backbone

pDONR223

backbone_mutation

backbone_origin

Invitrogen

backbone_size

2790

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

Gateway Entry vector

growth notes :

Open state: NO stop codon immediately follows insert. Supporting reference: Systematic morphological profiling of human gene and allele function via Cell Painting. Rohban MH, et al. Elife. 2017, Mar 18;6. pii: e24060. PubMed PMID 28315521

growth strain :

Gateway Donor vector containing PIK3CD , part of the Target Accelerator Plasmid Collection.

origin :

pi :

alt_names

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter	None
sequencing_primer_3	M13-Rev
sequencing_primer_5	M13pUC-fwd
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	APDS, IMD14, IMD14A, IMD14B, P110DELTA, PI3K, ROCHIS, p110D
gene	PIK3CD
id	5293

genbank_ids

NM_005026.3

mutation

name

PIK3CD

shRNA_sequence

size

species

9606

Homo sapiens