pDONR223_NRAS_WT | Addgene 82151 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pDONR223_NRAS_WT

catalog :

82151

citations: 2

Reference
Zhang H, Nakauchi Y, Köhnke T, Stafford M, Bottomly D, Thomas R, et al. Integrated analysis of patient samples identifies biomarkers for venetoclax efficacy and combination strategies in acute myeloid leukemia. Nat Cancer. 2020;1:826-839 pubmed publisher
Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, et al. Systematic Functional Interrogation of Rare Cancer Variants Identifies Oncogenic Alleles. Cancer Discov. 2016;6:714-26 pubmed publisher

product information

Catalog Number :

82151

Product Name :

pDONR223_NRAS_WT

article :

doi	10.1158/2159-8290.CD-16-0160
id	22007
pubmed_id	27147599

bacterial resistance :

Spectinomycin

cloning :

backbone

pDONR223

backbone_mutation

backbone_origin

Invitrogen

backbone_size

2790

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

Gateway Entry vector

growth notes :

Closed state: stop codon immediately follows insert Supporting reference: Systematic morphological profiling of human gene and allele function via Cell Painting. Rohban MH, et al. Elife. 2017, Mar 18;6. pii: e24060. PubMed PMID 28315521

growth strain :

Gateway Donor vector containing NRAS , part of the Target Accelerator Plasmid Collection.

origin :

pi :

alt_names

NM_002524.4

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter	None
sequencing_primer_3	M13-Rev
sequencing_primer_5	M13pUC-fwd
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ALPS4, CMNS, KRAS, N-ras, NCMS, NRAS1, NS6
gene	NRAS
id	4893

genbank_ids

mutation

name

NRAS

shRNA_sequence

size

species

9606

Homo sapiens