This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDONR223_TNF_WT
catalog :
81824
citations: 2
Reference
Ukrainskaya V, Bobik T, Argentova Stevens A, Slutskaya E, Kalinin R, Gabibov A, et al. Directed Change in TNFα Specificity to Create DR5 Antagonists. Bull Exp Biol Med. 2018;165:386-389 pubmed publisher
Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, et al. Systematic Functional Interrogation of Rare Cancer Variants Identifies Oncogenic Alleles. Cancer Discov. 2016;6:714-26 pubmed publisher
product information
Catalog Number :
81824
Product Name :
pDONR223_TNF_WT
article :
doi10.1158/2159-8290.CD-16-0160
id22007
pubmed_id27147599
bacterial resistance :
Spectinomycin
cloning :
backbonepDONR223
backbone_mutation
backbone_originInvitrogen
backbone_size2790
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Gateway Entry vector
growth notes :
Closed state: stop codon immediately follows insert
growth strain :
Gateway Donor vector containing TNF , part of the Target Accelerator Plasmid Collection.
origin :
37
pi :
alt_names
NM_000594.3
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterNone
sequencing_primer_3M13-Rev
sequencing_primer_5M13pUC-fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDIF, TNF-alpha, TNFA, TNFSF2, TNLG1F
geneTNF
id7124
genbank_ids
mutationWT
nameTNF
shRNA_sequence
size
species
9606
Homo sapiens
tags
resistance markers :
2996
184
423
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA