This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
PB53x EF1-Dendra2-RPB1Amr
catalog :
81228
citations: 1
Reference
Cho W, Jayanth N, English B, Inoue T, Andrews J, Conway W, et al. RNA Polymerase II cluster dynamics predict mRNA output in living cells. elife. 2016;5: pubmed publisher
product information
Catalog Number :
81228
Product Name :
PB53x EF1-Dendra2-RPB1Amr
article :
doi10.7554/eLife.13617
id21997
pubmed_id27138339
bacterial resistance :
Ampicillin
cloning :
backbonepb53
backbone_mutation
backbone_originSystem Biosciences
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
expresses Dendra2-Rpb1 fusion in mammalian cells. Possible to insert into the genome using the Piggibac system
origin :
37
pi :
alt_names
RPOLR2A, pIILS, POLRA, hsRPB1, POLR2, RPB1, RPBh1, RPOL2, RPO2, hRPB220
cloning
clone_methodRestriction Enzyme
cloning_site_3Unknown
cloning_site_5AgeI
promoterEF1a
sequencing_primer_3M13_pUC_fwd_primer
sequencing_primer_5M13_pUC_rev_primer
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesNEDHIB, POLR2, POLRA, RPB1, RPBh1, RPO2, RPOL2, RpIILS, hRPB220, hsRPB1
genePOLR2A
id5430
genbank_ids
mutationN792D alpha amanitin resistance mutation (Bartolomei & Corden Mol Gen Genet 1995)
nameDendra2 - Rpb1 (alpha amanitin resistant)
shRNA_sequence
size6698
species
9606
Homo sapiens
tags
locationN terminal on insert
tagDendra2
resistance markers :
2450
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA