This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRetro-XT-PAK2-CFP(puro)-L107F
catalog :
81180
citations: 1
Reference
Whalley H, Porter A, Diamantopoulou Z, White G, Castañeda Saucedo E, Malliri A. Cdk1 phosphorylates the Rac activator Tiam1 to activate centrosomal Pak and promote mitotic spindle formation. Nat Commun. 2015;6:7437 pubmed publisher
product information
Catalog Number :
81180
Product Name :
pRetro-XT-PAK2-CFP(puro)-L107F
article :
doi10.1038/ncomms8437
id20421
pubmed_id26078008
bacterial resistance :
Ampicillin
cloning :
backbonepRetroX-Tight-Pur
backbone_mutation
backbone_originClontech
backbone_size6567
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Retroviral
growth notes :
There is a coding difference of L106F rather than L107F in the PAK2 insert compared to NP_002568.2.
growth strain :
Retroviral vector for expression of kinase active PAK2 with a CFP fusion
origin :
30
pi :
alt_names
PAK2
cloning
clone_methodRestriction Enzyme
cloning_site_3NruI
cloning_site_5NotI
promoterP-tight TRE
sequencing_primer_3MSCV-rev
sequencing_primer_5pBABE 5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesKNO2, PAK65, PAKgamma
genePAK2
id5062
genbank_ids
mutationL107F
namep21 (RAC1) activated kinase 2
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tagCFP
resistance markers :
2995
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA