pSicoR-sh4EBP2 | Addgene 81121 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pSicoR-sh4EBP2

catalog :

81121

citations: 1

Reference
Hartman N, Lin T, Zhang L, Paquelet G, Feliciano D, Bordey A. mTORC1 targets the translational repressor 4E-BP2, but not S6 kinase 1/2, to regulate neural stem cell self-renewal in vivo. Cell Rep. 2013;5:433-44 pubmed publisher

product information

Catalog Number :

81121

Product Name :

pSicoR-sh4EBP2

article :

doi	10.1016/j.celrep.2013.09.017
id	12932
pubmed_id	24139800

bacterial resistance :

Ampicillin

cloning :

backbone

pSicoR

backbone_mutation

backbone_origin

Jacks Lab (MIT)

backbone_size

7567

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

RNAi

Cre/Lox

growth notes :

pSicoR allows for expression of the 4E-BP2 shRNA in the absence of Cre. Expression of Cre turns off shRNA expression. These plasmids were also used in the following publication Normalizing translation through 4E-BP prevents mTOR-driven cortical mislamination and ameliorates aberrant neuron integration. Lin TV, Hsieh L, Kimura T, Malone TJ, Bordey A. Proc Natl Acad Sci U S A. 2016 Oct 4;113(40):11330-11335.

growth strain :

expresses an shRNA targeting murine 4E-BP2

origin :

pi :

alt_names

Eif4ebp2 shRNA

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	HpaI
promoter	U6
sequencing_primer_3
sequencing_primer_5	mU6-F
site_3_destroyed
site_5_destroyed	1

entrez_gene

aliases	2810011I19Rik, 4E-BP2, AA792569, BC010348, PHAS-II
gene	Eif4ebp2
id	13688

genbank_ids

mutation

name

4E-BP2 shRNA

shRNA_sequence

CGCCTTAATTGAAGACTCCAA

size

species

10090

Mus musculus