This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
SIRT5-pET15b
catalog :
81030
citations: 1
Reference
Kumar S, Lombard D. Generation and Purification of Catalytically Active Recombinant Sirtuin5 (SIRT5) Protein. Methods Mol Biol. 2016;1436:241-57 pubmed publisher
product information
Catalog Number :
81030
Product Name :
SIRT5-pET15b
article :
doi10.1007/978-1-4939-3667-0_16
id21951
pubmed_id27246219
bacterial resistance :
Ampicillin
cloning :
backbonepET15b
backbone_mutation
backbone_originNovagen
backbone_size5708
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
The insert doesn't have mitochondrial targeting sequence.
growth strain :
Bacterial expression of Human WT SIRT5 protein
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NdeI
promoterT7
sequencing_primer_3T7 terminator
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesSIR2L5
geneSIRT5
id23408
genbank_ids
23408
mutation
nameSirt5
shRNA_sequence
size825
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHis
plasmid copy :
Drs. Marcia Haigis and Natalie German, Harvard Medical School
resistance markers :
2988
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA