This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
mPiezo1-IRES-eGFP
catalog :
80925
citations: 9
Reference
Mikesell A, Isaeva E, Schulte M, Menzel A, Sriram A, Prahl M, et al. Keratinocyte Piezo1 drives paclitaxel-induced mechanical hypersensitivity. bioRxiv. 2023;: pubmed publisher
Mulhall E, Gharpure A, Lee R, Dubin A, Aaron J, Marshall K, et al. Direct observation of the conformational states of PIEZO1. Nature. 2023;620:1117-1125 pubmed publisher
Chuntharpursat Bon E, Povstyan O, Ludlow M, Carrier D, Debant M, Shi J, et al. PIEZO1 and PECAM1 interact at cell-cell junctions and partner in endothelial force sensing. Commun Biol. 2023;6:358 pubmed publisher
Peralta F, Balcon M, Martz A, Biljali D, Cevoli F, Arnould B, et al. Optical control of PIEZO1 channels. Nat Commun. 2023;14:1269 pubmed publisher
Gong J, Chen J, Gu P, Shang Y, Ruppell K, Yang Y, et al. Shear stress activates nociceptors to drive Drosophila mechanical nociception. Neuron. 2022;110:3727-3742.e8 pubmed publisher
Li X, Han L, Nookaew I, Mannen E, Silva M, Almeida M, et al. Stimulation of Piezo1 by mechanical signals promotes bone anabolism. elife. 2019;8: pubmed publisher
Zheng W, Gracheva E, Bagriantsev S. A hydrophobic gate in the inner pore helix is the major determinant of inactivation in mechanosensitive Piezo channels. elife. 2019;8: pubmed publisher
Tsuchiya M, Hara Y, Okuda M, Itoh K, Nishioka R, Shiomi A, et al. Cell surface flip-flop of phosphatidylserine is critical for PIEZO1-mediated myotube formation. Nat Commun. 2018;9:2049 pubmed publisher
Coste B, Mathur J, Schmidt M, Earley T, Ranade S, Petrus M, et al. Piezo1 and Piezo2 are essential components of distinct mechanically activated cation channels. Science. 2010;330:55-60 pubmed publisher
product information
Catalog Number :
80925
Product Name :
mPiezo1-IRES-eGFP
article :
doi10.1126/science.1193270
id21920
pubmed_id20813920
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1
backbone_mutationIres-eGFP inserted
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
expresses mouse Piezo1 with GFP
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5NotI
promoter
sequencing_primer_3
sequencing_primer_5cgcaaatgggcggtaggcgtg
site_3_destroyed1
site_5_destroyed
entrez_gene
aliases9630020g22, Fam38a, mKIAA0233
genePiezo1
id234839
genbank_ids
mutation
namemouse Piezo1 CDS
shRNA_sequence
size7644
species
10090
Mus musculus
tags
resistance markers :
2990
tags :
Low Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA