This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-Flag-Smad4 R100T
catalog :
80891
citations: 2
Reference
Li J, Sun Y, Chen W, Fan J, Li S, Qu X, et al. Smad4 promotes diabetic nephropathy by modulating glycolysis and OXPHOS. EMBO Rep. 2020;21:e48781 pubmed publisher
Moren A, Itoh S, Moustakas A, Dijke P, Heldin C. Functional consequences of tumorigenic missense mutations in the amino-terminal domain of Smad4. Oncogene. 2000;19:4396-404 pubmed
product information
Catalog Number :
80891
Product Name :
pcDNA3-Flag-Smad4 R100T
article :
doi10.1038/sj.onc.1203798
id21911
pubmed_id10980615
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
This plasmid contains a D2E mutation in SMAD4 compared to NM_005359.5. The depositor states that this mutation does not affect plasmid function.
growth strain :
mammalian expression of Smad4 R100T
origin :
37
pi :
alt_names
SMAD family member 4
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5EcoRI
promoterCMV
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDPC4, JIP, MADH4, MYHRS
geneSMAD4
id4089
genbank_ids
mutationPancreatic cancer mutation R100T
nameSmad4
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagFlag
resistance markers :
2976
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA