pcDNA3-Flag-Smad4 | Addgene 80888 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA3-Flag-Smad4

catalog :

80888

citations: 4

Reference
Li J, Sun Y, Chen W, Fan J, Li S, Qu X, et al. Smad4 promotes diabetic nephropathy by modulating glycolysis and OXPHOS. EMBO Rep. 2020;21:e48781 pubmed publisher
Wang F, Xia X, Yang C, Shen J, Mai J, Kim H, et al. SMAD4 Gene Mutation Renders Pancreatic Cancer Resistance to Radiotherapy through Promotion of Autophagy. Clin Cancer Res. 2018;24:3176-3185 pubmed publisher
Hopkins A, Coatham M, Berry F. FOXC1 Regulates FGFR1 Isoform Switching to Promote Invasion Following TGF?-Induced EMT. Mol Cancer Res. 2017;15:1341-1353 pubmed publisher
Moren A, Itoh S, Moustakas A, Dijke P, Heldin C. Functional consequences of tumorigenic missense mutations in the amino-terminal domain of Smad4. Oncogene. 2000;19:4396-404 pubmed

product information

Catalog Number :

80888

Product Name :

pcDNA3-Flag-Smad4

article :

doi	10.1038/sj.onc.1203798
id	21911
pubmed_id	10980615

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

The Smad4 insert contains a D2E mutation. The depositor states that this mutation does not affect plasmid function.

growth strain :

mammalian expression of Smad4

origin :

pi :

alt_names

SMAD family member 4

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	EcoRI
promoter	CMV
sequencing_primer_3	BGH-rev
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	DPC4, JIP, MADH4, MYHRS
gene	SMAD4
id	4089

genbank_ids

mutation

name

Smad4

shRNA_sequence

size

species

9606

Homo sapiens