This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pE-SUMO CRYBB3
catalog :
80750
product information
Catalog Number :
80750
Product Name :
pE-SUMO CRYBB3
article :
doi
id18889
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepE-SUMO
backbone_mutation
backbone_originLifeSensors
backbone_size5761
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Expresses well as a N-terminal SUMO tagged protein with a 6XHis tag on the N-terminus for purification. The SUMO tag can be removed using ulp1 SUMO protease to yield the N-terminal Met of mature beta B3-cystallin. Both the SUMO tag and ulp1 SUMO protease can then be removed using an NTA resin. ulp1 SUMO protease can be ordered from LifeSensors or expressed using AddGene plasmid 64697.
growth strain :
Expression of human beta B3-crystallin in E. coli
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5BsaI
promoterT7
sequencing_primer_3T7 terminator
sequencing_primer_5T7
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesCATCN2, CRYB3, CTRCT22
geneCRYBB3
id1417
genbank_ids
CR456427
mutation
nameCRYBB3
shRNA_sequence
size633
species
9606
Homo sapiens
tags
locationN terminal on insert
tag6xHIS
locationN terminal on insert
tagSmt3 SUMO
plasmid copy :
DNASU plasmid HsCD00620837
resistance markers :
2965
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA