NLS-AgDD | Addgene 80625 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

NLS-AgDD

catalog :

80625

citations: 3

Reference
Li D, Johmura Y, Morimoto S, Doi M, Nakanishi K, Ozawa M, et al. LONRF2 is a protein quality control ubiquitin ligase whose deficiency causes late-onset neurological deficits. Nat Aging. 2023;: pubmed publisher
Schellhaus A, Xu S, Gierisch M, Vornberger J, Johansson J, Dantuma N. A spider silk-derived solubility domain inhibits nuclear and cytosolic protein aggregation in human cells. Commun Biol. 2022;5:505 pubmed publisher
Miyazaki Y, Mizumoto K, Dey G, Kudo T, Perrino J, Chen L, et al. A method to rapidly create protein aggregates in living cells. Nat Commun. 2016;7:11689 pubmed publisher

product information

Catalog Number :

80625

Product Name :

NLS-AgDD

article :

doi	10.1038/ncomms11689
id	18647
pubmed_id	27229621

bacterial resistance :

Ampicillin

cloning :

backbone

PiggyBac transformation vector pB

backbone_mutation

backbone_origin

backbone_size

6652

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Depositor reference sequence and Addgene Sanger results show F36V compared to NM_000801.4.

growth strain :

create nuclear protein aggregates in mammalian cells

origin :

pi :

alt_names

NLS-AgDD

cloning

clone_method	Restriction Enzyme
cloning_site_3	HpaI
cloning_site_5	EcoRI
promoter
sequencing_primer_3	YM215--5' gatgagtttggacaaaccacaact
sequencing_primer_5	YM213--5' atgccttcttctttttcctacagct
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	FKBP-12, FKBP-1A, FKBP1, FKBP12, PKC12, PKCI2, PPIASE
gene	FKBP1A
id	2280

genbank_ids

NM_000801

AB971579

mutation

name

NLS-NES-L106P-sfGFP

shRNA_sequence

size

1113

species

9606

Homo sapiens

32644

Other