PB-TAC-EB-C5 | Addgene 80485 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

PB-TAC-EB-C5

catalog :

80485

citations: 1

Reference
Kim S, Oceguera Yanez F, Hirohata R, Linker S, Okita K, Yamada Y, et al. KLF4 N-terminal variance modulates induced reprogramming to pluripotency. Stem Cell Reports. 2015;4:727-43 pubmed publisher

product information

Catalog Number :

80485

Product Name :

PB-TAC-EB-C5

article :

doi	10.1016/j.stemcr.2015.02.004
id	18839
pubmed_id	25772473

bacterial resistance :

Ampicillin

cloning :

backbone

pBluescript

backbone_mutation

backbone_origin

backbone_size

3000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Other

piggyBac transposon

growth notes :

Requires co-transfection with a piggyBac transposase plasmid for stable genomic integration. If you desire to use transposase with the Materials, you may contact Transposagen or other licensed vendor.

growth strain :

piggyBac transposon for dox-inducible expression of the EB-C5 polycistronic reprogramming cassette (with mCherry)

origin :

pi :

alt_names

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter	tetO
sequencing_primer_3	GCTGTTTTGACCTCCATAGAAGA
sequencing_primer_5	CCAAAAGACGGCAATATGGT
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	NF-A3, Oct-3, Oct-3/4, Oct-4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4
gene	Pou5f1
id	18999

aliases	Sox-2, lcc, ysb
gene	Sox2
id	20674

aliases	EZF, Gklf, Zie
gene	Klf4
id	16600

aliases	Myc2, Niard, Nird, bHLHe39
gene	Myc
id	17869

aliases	Gm10299, Lin-28, Lin28, Tex17, lin-28A
gene	Lin28a
id	83557

genbank_ids

mutation

name

lin-28 homolog A (C. elegans)

shRNA_sequence

size

5676

species

10090

Mus musculus