This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMV6-Neo-ISG15-LRAA
catalog :
80405
citations: 1
Reference
Gonzalez Sanz R, Mata M, Bermejo Martín J, Alvarez A, Cortijo J, Melero J, et al. ISG15 Is Upregulated in Respiratory Syncytial Virus Infection and Reduces Virus Growth through Protein ISGylation. J Virol. 2016;90:3428-38 pubmed publisher
product information
Catalog Number :
80405
Product Name :
pCMV6-Neo-ISG15-LRAA
article :
doi10.1128/JVI.02695-15
id18811
pubmed_id26763998
bacterial resistance :
Ampicillin
cloning :
backbonepCMV6-Neo
backbone_mutation
backbone_originOrigene
backbone_size5835
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Synthesis of the nonconjugative ISG15 plasmid pCMV6-Neo-ISG15-LRAA from pCMV6-Neo-ISG15 (Addgene plasmid 80404) was performed by directed mutagenesis using the Phusion site-directed mutagenesis kit (Thermo Scientific), following the manufacturer s instructions, with the following primers: forward, (5 CCTGCGGGCAGCCGGCACAGAGCCTGGCGGGCGGAGC3 ), and reverse, (5 GGCTGCCCGCAGGCGCAGATTCATGAACACGGT3 ).
growth strain :
overexpression of nonconjugative ISG15
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5NotI
promoterCMV
sequencing_primer_3hGH-PA-R
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesG1P2, IFI15, IMD38, IP17, UCRP, hUCRP
geneISG15
id9636
genbank_ids
NM_005101.3
mutationnonconjugative ISG15, LRAA
nameISG15
shRNA_sequence
size685
species
9606
Homo sapiens
tags
resistance markers :
2942
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA