pEGFP-VSP4-E228Q | Addgene 80351 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pEGFP-VSP4-E228Q

catalog :

80351

citations: 4

Reference
Lee J, Xu Y, Saidi L, Xu M, ZINSMAIER K, Ye Y. Abnormal triaging of misfolded proteins by adult neuronal ceroid lipofuscinosis-associated DNAJC5/CSPα mutants causes lipofuscin accumulation. Autophagy. 2022;:1-20 pubmed publisher
Rheinemann L, Downhour D, Bredbenner K, Mercenne G, Davenport K, Schmitt P, et al. RetroCHMP3 blocks budding of enveloped viruses without blocking cytokinesis. Cell. 2021;184:5419-5431.e16 pubmed publisher
Coyne A, Rothstein J. The ESCRT-III protein VPS4, but not CHMP4B or CHMP2B, is pathologically increased in familial and sporadic ALS neuronal nuclei. Acta Neuropathol Commun. 2021;9:127 pubmed publisher
Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap D, et al. Designed proteins induce the formation of nanocage-containing extracellular vesicles. Nature. 2016;540:292-295 pubmed publisher

product information

Catalog Number :

80351

Product Name :

pEGFP-VSP4-E228Q

article :

doi	10.1038/nature20607
id	23059
pubmed_id	27919066

bacterial resistance :

Kanamycin

cloning :

backbone

pEGFP-C1

backbone_mutation

backbone_origin

Clonetech

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

Expression of GFP-tagged, dominant negative VPS4 mutant

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	BamHI
cloning_site_5	EcoRI
promoter	CMV
sequencing_primer_3
sequencing_primer_5	CMV-Forward
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CIMDAG, SKD1, SKD1A, SKD2, VPS4, VPS4-1
gene	VPS4A
id	27183

genbank_ids

mutation

name

VPS4-E228Q

shRNA_sequence

size

1353

species

9606

Homo sapiens