pcDNA4/TO/FLAG-hPMR1(2H)-Tev-Bio | Addgene 80325 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA4/TO/FLAG-hPMR1(2H)-Tev-Bio

catalog :

80325

citations: 1

Reference
Gu S, Bakthavachalu B, Han J, Patil D, Otsuka Y, Guda C, et al. Identification of the human PMR1 mRNA endonuclease as an alternatively processed product of the gene for peroxidasin-like protein. RNA. 2012;18:1186-96 pubmed publisher

product information

Catalog Number :

80325

Product Name :

pcDNA4/TO/FLAG-hPMR1(2H)-Tev-Bio

article :

doi	10.1261/rna.031369.111
id	11620
pubmed_id	22543864

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA4/TO

backbone_mutation

backbone_origin

Thermo Fisher Scientific

backbone_size

4987

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

hPMR1 corresponds to Ensembl transcript PXDNL-003. In this form of the protein the 2 histidine residues that correspond to the active site have been changed to alanine to generate a catalytically inactive protein. Please note that the hPMR1 insert in this plasmid is numbered starting with the first methionine in GenBank reference sequence BC132813. This plasmid does not encode the 1463 aa PXDNL protein in GenBank NM_144651.4

growth strain :

Expresses a catalytically inactive form of human PMR1 endonuclease with N-terminal FLAG and C-terminal biotinylation tags

origin :

pi :

alt_names

hPMR1

cloning

clone_method	Restriction Enzyme
cloning_site_3	ApaI
cloning_site_5	HindIII
promoter	CMV
sequencing_primer_3	ggcgtccctgagccgggtgctttgtg
sequencing_primer_5	cggcgtgccgggtgttcatgggg
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	PMR1, PRM1, VPO2
gene	PXDNL
id	137902

genbank_ids

BC132813

mutation

H170A and H256A

name

PXDNL-003

shRNA_sequence

size

1910

species

9606

Homo sapiens