This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA4/TO/FLAG-hPMR1-Tev-Bio
catalog :
80125
citations: 1
Reference
Gu S, Bakthavachalu B, Han J, Patil D, Otsuka Y, Guda C, et al. Identification of the human PMR1 mRNA endonuclease as an alternatively processed product of the gene for peroxidasin-like protein. RNA. 2012;18:1186-96 pubmed publisher
product information
Catalog Number :
80125
Product Name :
pcDNA4/TO/FLAG-hPMR1-Tev-Bio
article :
doi10.1261/rna.031369.111
id11620
pubmed_id22543864
bacterial resistance :
Ampicillin
cloning :
backbonepCDNA4/TO
backbone_mutation
backbone_originThermo Fisher Scientific
backbone_size5078
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
hPMR1 corresponds to Ensembl transcript PXDNL-003 Please note that the hPMR1 insert in this plasmid is numbered starting with the first methionine in GenBank reference sequence BC132813. This plasmid does not encode the 1463 aa PXDNL protein in GenBank NM_144651.4
growth strain :
Expresses human PMR1 endonuclease with N-terminal FLAG and C-terminal biotinylation tags
origin :
37
pi :
alt_names
hPMR1
cloning
clone_methodRestriction Enzyme
cloning_site_3ApaI
cloning_site_5HindIII
promoterCMV promoter
sequencing_primer_3ggcgtccctgagccgggtgctttgtg
sequencing_primer_5cggcgtgccgggtgttcatgggg
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesPMR1, PRM1, VPO2
genePXDNL
id137902
genbank_ids
BC132813
mutation
namePXDNL-003
shRNA_sequence
size1910
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFLAG
locationC terminal on insert
tagbiotinylation
plasmid copy :
Open Biosystems (catalog no. MHS 4426-99240274)
resistance markers :
2937
tags :
High Copy
terms :
Zeocin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA