This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
GFP-BLM
catalog :
80070
citations: 6
Reference
Liu S, Atkinson E, Paulucci Holthauzen A, Wang B. A CK2 and SUMO-dependent, PML NB-involved regulatory mechanism controlling BLM ubiquitination and G-quadruplex resolution. Nat Commun. 2023;14:6111 pubmed publisher
Mason Osann E, Terranova K, Lupo N, Lock Y, Carson L, Flynn R. RAD54 promotes alternative lengthening of telomeres by mediating branch migration. EMBO Rep. 2020;21:e49495 pubmed publisher
Loe T, Li J, Zhang Y, Azeroglu B, BODDY M, Denchi E. Telomere length heterogeneity in ALT cells is maintained by PML-dependent localization of the BTR complex to telomeres. Genes Dev. 2020;34:650-662 pubmed publisher
Panier S, Maric M, Hewitt G, Mason Osann E, Gali H, Dai A, et al. SLX4IP Antagonizes Promiscuous BLM Activity during ALT Maintenance. Mol Cell. 2019;76:27-43.e11 pubmed publisher
Patel D, Misenko S, Her J, Bunting S. BLM helicase regulates DNA repair by counteracting RAD51 loading at DNA double-strand break sites. J Cell Biol. 2017;216:3521-3534 pubmed publisher
Hu P, Beresten S, van Brabant A, Ye T, Pandolfi P, Johnson F, et al. Evidence for BLM and Topoisomerase IIIalpha interaction in genomic stability. Hum Mol Genet. 2001;10:1287-98 pubmed
product information
Catalog Number :
80070
Product Name :
GFP-BLM
article :
doi
id18738
pubmed_id11406610
bacterial resistance :
Kanamycin
cloning :
backboneEGFP-C1
backbone_mutationSalI cloning site duplicated. No other changes
backbone_originCLONTECH
backbone_size4731
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses GFP-tagged BLM helicase in mammalian cells (EGFP-C1 backbone)
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3none
cloning_site_5SalI
promoter
sequencing_primer_3not sure
sequencing_primer_5not sure
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBS, MGRISCE1, RECQ2, RECQL2, RECQL3
geneBLM
id641
genbank_ids
U39817.1
NP_000048.1
mutationIf correct ID, the cDNA is from HeLa and has characteristic African-ancestry nt
nameBLM
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tagGFP
resistance markers :
2922
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA