This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMTB2-RanGAP-Cerulean-Avi
catalog :
80068
citations: 1
Reference
Trinh L, Chong Morrison V, Gavriouchkina D, Hochgreb Hägele T, Senanayake U, Fraser S, et al. Biotagging of Specific Cell Populations in Zebrafish Reveals Gene Regulatory Logic Encoded in the Nuclear Transcriptome. Cell Rep. 2017;19:425-440 pubmed publisher
product information
Catalog Number :
80068
Product Name :
pMTB2-RanGAP-Cerulean-Avi
article :
doi10.1016/j.celrep.2017.03.045
id25600
pubmed_id28402863
bacterial resistance :
Ampicillin
cloning :
backbonepMT
backbone_mutation
backbone_origin
backbone_size6332
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
Other
zebrafish biotagging
growth strain :
Beta-actin2 promoter driving C-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequences
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterBeta-actin2
sequencing_primer_3taatacgactcactatagggag
sequencing_primer_5atttaggtgacactatagaagag
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneRANGAP1
id417997
genbank_ids
NM_001006237.1
mutation
nameCerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1)
shRNA_sequence
size1360
species
9031
Gallus gallus
tags
locationC terminal on insert
tagAvi
resistance markers :
2979
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA