This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pX330-COSMC-KO
catalog :
80008
citations: 1
Reference
Stolfa G, Mondal N, Zhu Y, Yu X, Buffone A, Neelamegham S. Using CRISPR-Cas9 to quantify the contributions of O-glycans, N-glycans and Glycosphingolipids to human leukocyte-endothelium adhesion. Sci Rep. 2016;6:30392 pubmed publisher
product information
Catalog Number :
80008
Product Name :
pX330-COSMC-KO
article :
doi10.1038/srep30392
id21991
pubmed_id27458028
bacterial resistance :
Ampicillin
cloning :
backbonepX330-U6-Chimeric_BB-CBh-hSpCas9
backbone_mutationGuide RNA for COSMC gene cloned in BbsI restriction site
backbone_origin
backbone_size8506
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
CRISPR
growth strain :
gRNA to knock out expression of COSMC (C1GalT1C1) gene. The product of this gene is a chaperone aiding in synthesis of Core1 structures on O-linked glycans.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BbsI
cloning_site_5BbsI
promoter
sequencing_primer_3
sequencing_primer_5LKO.1 5'
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesC1GALT2, C38H2-L1, COSMC, HSPC067, MST143, TNPS
geneC1GALT1C1
id29071
genbank_ids
mutation
nameCOSMC
shRNA_sequenceGAGTCTTTGGGCTGCAGTAA
size
species
9606
Homo sapiens
tags
plasmid copy :
Cloning vector, pX330-U6-Chimeric_BB-CBh-hSpCas9 (Addgene 42230), was produced in Dr. Feng Zhang's lab.
resistance markers :
2323
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA