This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMTB2-Avi-Cerulean-Rpl10a
catalog :
79880
citations: 1
Reference
Trinh L, Chong Morrison V, Gavriouchkina D, Hochgreb Hägele T, Senanayake U, Fraser S, et al. Biotagging of Specific Cell Populations in Zebrafish Reveals Gene Regulatory Logic Encoded in the Nuclear Transcriptome. Cell Rep. 2017;19:425-440 pubmed publisher
product information
Catalog Number :
79880
Product Name :
pMTB2-Avi-Cerulean-Rpl10a
article :
doi10.1016/j.celrep.2017.03.045
id25600
pubmed_id28402863
bacterial resistance :
Ampicillin
cloning :
backbonepMT
backbone_mutation
backbone_origin
backbone_size6084
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Beta-actin2 promoter driving Avi-tagged protein containing Cerulean protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 2012); flanked by Tol2 sequences
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterBeta-actin2
sequencing_primer_3taatacgactcactatagggag
sequencing_primer_5atttaggtgacactatagaagag
site_3_destroyed
site_5_destroyed
entrez_gene
aliaseswu:fb94f08, zgc:73082, zgc:86881
generpl10a
id323305
genbank_ids
mutation
nameCerulean protein fused to zebrafish Rpl10a ribosomal unit
shRNA_sequence
size1759
species
7955
Danio rerio
tags
locationN terminal on insert
tagAvi
plasmid copy :
Rpl10a ORF (648 bp) obtained from Tol2-zTRAP (Addgene plasmid 42236, Tryon et al. 2012)
resistance markers :
2979
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA