RP4-11 | Addgene 79812 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

RP4-11

catalog :

79812

citations: 1

Reference
Quandt J, Clark R, Venter A, Clark S, Twelker S, Hynes M. Modified RP4 and Tn5-Mob derivatives for facilitated manipulation of large plasmids in Gram-negative bacteria. Plasmid. 2004;52:1-12 pubmed

product information

Catalog Number :

79812

Product Name :

RP4-11

article :

doi	10.1016/j.plasmid.2004.04.002
id	18685
pubmed_id	15212888

bacterial resistance :

Kanamycin

cloning :

backbone

RP4

backbone_mutation

Insertion of KmSacB cassette by in vivo recombination. For details see original Quandt et al. paper from 2004.

backbone_origin

natural plasmid

backbone_size

60000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

Helper plasmid for mobilization

growth notes :

This plasmid is designed to be used in mobilizing other plasmids, and is easily curable from recipient cells because of the sacB gene. Depositor states that growth on the respective antibiotic is sufficient to confirm plasmid integrity. Depositor states that users can select for the chromosome with nalidixic acid.

growth strain :

Modified RP4 plasmid with Km resistance and sacB gene. Easily curable helper plasmid

growth temp :

Select for the chromosome with nalidixic acid.

inserts :

CSH56

origin :

pi :

alt_names

Km, SacB

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter	Km
sequencing_primer_3	None
sequencing_primer_5	None
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	BSU_34450, BSU34450
gene	sacB
id	936413

genbank_ids

mutation

name

Kanamycin cassette and SacB gene

shRNA_sequence

size

2800

species