This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Lambda Phosphatase
catalog :
79748
citations: 4
Reference
Lu x10d i x107 I, H xe9 luin L, Jiang P, Castro Scalise A, Wang C, Franz A, et al. CaMKII autophosphorylation can occur between holoenzymes without subunit exchange. elife. 2023;12: pubmed publisher
Park J, Jeon H, Shin S, Song C, Lee H, Kim N, et al. Structural basis for CEP192-mediated regulation of centrosomal AURKA. Sci Adv. 2023;9:eadf8582 pubmed publisher
Budziszewski G, Zhao Y, Spangler C, Kedziora K, Williams M, Azzam D, et al. Multivalent DNA and nucleosome acidic patch interactions specify VRK1 mitotic localization and activity. Nucleic Acids Res. 2022;50:4355-4371 pubmed publisher
Albanese S, Parton D, Işık M, Rodríguez Laureano L, Hanson S, Behr J, et al. An Open Library of Human Kinase Domain Constructs for Automated Bacterial Expression. Biochemistry. 2018;57:4675-4689 pubmed publisher
product information
Catalog Number :
79748
Product Name :
Lambda Phosphatase
article :
doi10.1021/acs.biochem.7b01081
id18654
pubmed_id30004690
bacterial resistance :
Spectinomycin
cloning :
backbonepET co-transformation cloning vector (13S-A)
backbone_mutation
backbone_originScott Gradia (Addgene plasmid # 48323)
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
This plasmid was generated as part of an open library of human kinase domain constructs for automated bacterial expression. The work can be found here doi: http://dx.doi.org/10.1101/038711
growth strain :
Intended for co-expression with human Ser/Thr kinase plasmids to enhance bacterial kinase expression.
origin :
37
pi :
alt_names
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterT7
sequencing_primer_3T7 reverse
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameLambda Phosphatase
shRNA_sequence
size
species
32644
Other
tags
resistance markers :
2903
3190
3196
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA